| Catalog # | H5110-11G |
| Applications | Suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 0.1-0.2ug/ml. Detected monomethylated Histone H3 in acid extracted proteins from HeLa cells, but did not detect unmethylated recombinant Histone H3. Recombinant Histone H3 and acid extracts from HeLa cells were resolved by electrophoresis. transferred to nitrocellulose amd probed with H5110-11G (0.2mg/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.105M sodium chloride, 0.035% sodium azide before the addition of glycerol to 30%. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Synthetic 2X-branched peptide containing the sequence |
| …ARmeKST… in which meK corresponds to monomethyl-lysine at |
| residue 9 of human Histone H3 (KLH). |
| Specificity | Recognizes human monomethylated Histone H3 (Lys9), Mr ~17kD. Species Crossreactivity: mouse and chicken. Broad species crossreactivity is expected based on sequence homology. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
