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You are here:Home » Antibodies » Antibodies-Histones » Anti -Histone H3, monomethyl (Lys9)

Anti -Histone H3, monomethyl (Lys9)


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Clone Host Grade Applications
Polyclonal Rabbit Serum B
Catalog #H5110-11G2
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionImmunoblot Analysis: A 1:1000 dilution of this lot detected methylated Histone H3 in acid-extracted proteins from HeLa cells. The antibody did not detect unmethylated recombinant histone H3 purified from E. coli.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationNot determined
FormSupplied as a liquid containing 0.05% sodium azide and 30% glycerol.
PuritySerum depleted of nonmethyl cross-reactive antibodies
ImmunogenKLH-conjugated, synthetic peptide containing the sequence ...ARmmKS… in which mmK corresponds to monomethyl-lysine at residue 9 of human Histone H3. The immunizing sequence is conserved from Tetrahymena to human.
SpecificityRecognizes methylated Histone H3 (Lys9), Mr ~17kD. Specificity demonstrated by ELISA using mono-, di-, and unmethylated peptides. Species Crossreactivity: Human. A broad species crossreactivity is expected.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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