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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, monomethyl (Lys36)

Anti -Histone H3, monomethyl (Lys36)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IF Gel
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eurokaryotic cells. Featuring a main globuular domaina and a long N-terminal tail. H3 is involved with the structure of the nucleosomes of the “beads of string” structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Catalog #H5110-11N
ApplicationsSuitable for use in Western Blot, Immunofluorescence, Beadlyte Histone Peptide Assay and Peptide Dot Blot Analysis. Other applications not tested.
Recommended DilutionsWestern Blot: 1:500-1:2000 detected monomethyl-Histone H3 (Lys36) in chicken core histones. Did not detect unmodified recombinant Histone H3. Recombinant histone H3 and chicken core histones were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-11N. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Beadlyte Histone Peptide Assay: 1:10,000-1:80,000 detected only the histone H3 peptide containing the monomethyl lysine 36 modification
Peptide Dot Blot Analysis: 1:5000 detected only the histone H3 peptide containing the monomethyl lysine 36 modification. A dilution series of Histone H3 peptides.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 0.05% sodium azide, 30% glycerol.
PuritySerum
ImmunogenSynthetic peptide containing the sequence …GVmeKKP… in which meK corresponds to monomethyl Lysine 36 of human histone H3, conjugated to KLH.
SpecificityRecognizes human monomethyl-Histone H3 (Lys36), Mr ~17kD. A non-specific protein was also detected, Mr ~200kD. Broad species crossreactivity is expected.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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