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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, phosphorylated (Ser10)

Anti -Histone H3, phosphorylated (Ser10)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Affinity Purified B IC
Catalog #H5110-11Q
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:2,000-1:10,000 detects phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells treated with colcemid, but not unmodified recombinant histone H3. Recombinant histone H3 and acid extract from colcemid treated HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-Histone H3 (Ser10) (1:2,000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunocytochemistry 1:100-1:10,000 showed positive chromosome immunostaining for mitotic HeLa cells and A431 cells fixed with 95% ethanol and 5% acetic acid and permeabilized with 0.1% Triton-X 100.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No4H337
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide containing the sequence ...RK[pS]TG… in which pS corresponds to phospho-serine at position 10 of
human histone H3.
SpecificityRecognizes histone H3 phosphorylated at Ser10, Mr 17kD. Species Crossreactivity: Human. Broad species crossreactivity is expected.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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