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Specifications

Clone Host Grade Applications Polyclonal Sheep Affinity Purified B IP IH IC

Catalog #	H5110-12A6
Applications	Suitable for use in Western Blot, Immunocytochemistry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:300
	Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
	Immunocytochemistry: 1:100
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Sheep
Source	Bovine
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Intact calf histone H3 at 15.4kD, 136aa, complexed with RNA.
Specificity	Recognizes bovine Histone H3. Minimal cross-reactivity with linker histone H1 and no cross-reactivity with histone core proteins H2A, H2B, or H4. Species sequence homology: human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.