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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3

Anti -Histone H3

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B IP IH IC
Catalog #H5110-12A6
ApplicationsSuitable for use in Western Blot, Immunocytochemistry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:300
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Immunocytochemistry: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceBovine
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4.
PurityPurified by Protein G affinity chromatography.
ImmunogenIntact calf histone H3 at 15.4kD, 136aa, complexed with RNA.
SpecificityRecognizes bovine Histone H3. Minimal cross-reactivity with linker histone H1 and no cross-reactivity with histone core proteins H2A, H2B, or H4. Species sequence homology: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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