| Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various posttranslational modifications, including acetylation, phosphorylation, methylation and ubiquitination (2–5). These modifications occur in response to various stimuli and have a |
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| direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6). In most species, histone H2B is primarily acetylated at lysines 5, 12, 15 and 20 (4,7). Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23 (2,3). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8). |
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| Catalog # | H5110-12B |
| Applications | Suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:1000. MW: 17kD |
| Note: For Western Blots, incubate membrane with diluted antibody in 5% BSA, 1x TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Clone No | 5i118 |
| Host | Rabbit |
| Source | Human |
| Concentration | 100ul (10 mini Western blots) |
| Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol. |
| Purity | Serum |
| Immunogen | Synthetic peptide corresponding to the carboxy-terminal residues of human histone H3 (KLH-coupled) |
| Specificity | Detects endogenous levels of total human histone H3 protein. Does not crossreact with other proteins. Species Crossreactivity |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
