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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, phosphorylated (Ser10), acetylated (Ac-K14)

Anti -Histone H3, phosphorylated (Ser10), acetylated (Ac-K14)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IC
Catalog #H5110-12H
ApplicationsSuitable for use in Western Blotting, Immunoprecipitation, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blotting: 1:2000-1:5000
Immunocytochemistry: recommended
Immunoprecipitation (Chromatin): 1ug/mg of total cell protein (~10e7 cells)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
FormSupplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 5mg/ml BSA, before the addition of glycerol to 30%.
PurityPurified by immunoaffinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 7-20 of human histone H3 (ARK[pS]TGGAcKAPRKQL-C).
SpecificityRecognizes Histone H3 phosphorylated at serine 10 and acetylated at lysine 14, Mr 17kD. An unidenti ed protein is also seen in some cells lines, Mr 40kD. Species Crossreactivity: Human and mouse; broad species Crossreactivity expected.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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