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Anti -Histone H3, phosphorylated (Ser10), acetylated (Ac-K14)
Anti -Histone H3, phosphorylated (Ser10), acetylated (Ac-K14)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IP IC |
|
| Catalog # | H5110-12H | | Applications | Suitable for use in Western Blotting, Immunoprecipitation, Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blotting: 1:2000-1:5000 | | Immunocytochemistry: recommended | | Immunoprecipitation (Chromatin): 1ug/mg of total cell protein (~10e7 cells) | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Form | Supplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 5mg/ml BSA, before the addition of glycerol to 30%. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | KLH-conjugated, synthetic peptide corresponding to amino acids 7-20 of human histone H3 (ARK[pS]TGGAcKAPRKQL-C). | | Specificity | Recognizes Histone H3 phosphorylated at serine 10 and acetylated at lysine 14, Mr 17kD. An unidenti ed protein is also seen in some cells lines, Mr 40kD. Species Crossreactivity: Human and mouse; broad species Crossreactivity expected. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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