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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, phosphorylated (Ser28)

Anti -Histone H3, phosphorylated (Ser28)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IC
Catalog #H5110-12J
ApplicationsSuitable for use in Western Blotting, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot : 1:250-1:2000
Immunocytochemistry: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
FormSupplied as serum, 0.05% sodium azide, 30% glycerol.
PuritySerum
ImmunogenBSA-conjugated, synthetic peptide corresponding to amino acids 25-37 of human Histone H3 (ARK[pS]APATGGVKK-C), with a C-terminal cysteine added to facilitate conjugation.
SpecificityRecognizes Histone H3 phosphorylated at Ser28, Mr 17kD. BSA is observed, Mr 66kD, in longer exposures. Does not cross-react with Histone H3 phosphorylated at Serine 10. Species Crossreactivity: Human. Broad species Crossreactivity is expected.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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