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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, phosphorylated (Ser28)

Anti -Histone H3, phosphorylated (Ser28)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IC
Catalog #H5110-12J
ApplicationsSuitable for use in Western Blotting, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot : 1:250-1:2000
Immunocytochemistry: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Numbern/a
Clone TypePolyclonal
HostRabbit
SourceHuman
FormSupplied as serum, 0.05% sodium azide, 30% glycerol.
PuritySerum
ImmunogenBSA-conjugated, synthetic peptide corresponding to amino acids 25-37 of human Histone H3 (ARK[pS]APATGGVKK-C), with a C-terminal cysteine added to facilitate conjugation.
SpecificityRecognizes Histone H3 phosphorylated at Ser28, Mr 17kD. BSA is observed, Mr 66kD, in longer exposures. Does not cross-react with Histone H3 phosphorylated at Serine 10. Species Crossreactivity: Human. Broad species Crossreactivity is expected.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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