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You are here:Home » Antibodies » Antibodies-Histones » Anti -Histone H3, phosphorylated (Ser28)

Anti -Histone H3, phosphorylated (Ser28)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
The phosphorylation of histone H3 plays an important role in gene expression, chromatin remodeling, chromosome condensation and cell division. Histone H3 phosphorylation may initiate at different phases of the cell division in different organisms. Metaphase chromosomes are always found to be heavily phosphorylated. H3 phosphorylation at serine 28 is coupled with mitotic chromosome condensation in diverse mammalian cell lines
Catalog #H5110-12M
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 2ug/ml ~18kD band is detected only in treated cell lysate
Optimal dilution determined by the researcher.
Positive ControlAnti-Fas mAb treated Jurkat cell lysates.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No4H101
FormSupplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, before the addition of glycerol to 40%.
PurityPurified by protein G affinity chromatography.
ImmunogenSynthetic peptide containing phosphorylated serine at position 28 of human Histone H3.
SpecificityRecognizes human Histone H3, phosphorylated (pSer28)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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