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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, phosphorylated (Ser28)

Anti -Histone H3, phosphorylated (Ser28)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
The phosphorylation of histone H3 plays an important role in gene expression, chromatin remodeling, and chromosome condensation, and cell division. Histone H3 phosphorylation may initiate at different phases of the cell division in different organisms, but metaphase chromosomes are always found to be heavily phosphorylated. H3 phosphorylation at serine 28 is coupled with mitotic chromosome condensation in diverse mammalian cell lines.
Catalog #H5110-12M2
Positive Control anti-Fas mAb treated Jurkat
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8L222
HostMouse
SourceHuman
ConcentrationAs reported
FormContaining 0.2% gelatin, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic phosphopeptide around serine at position 28 [BLAST]CARKS*APATGGVKK[/BLAST] of human Histone H3 was used as immunogen. The immunogen sequence (CARKS*APATGGVKK) is conserved across mammalian and invertebrates, as well as plants.
SpecificityRecognizes Histone H3 when phosphorylated at Ser28. In anti-Fas mAb treated Jurkat, an 18kD band is observed. The antibody has been evaluated in human and is predicted to work accross mutliple species due to conservation of the sequence used for immunogen. Species Crossreactivity: Human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesH3, HisH3, His1H3, H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l


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