| The phosphorylation of histone H3 plays an important role in gene expression, chromatin remodeling, and chromosome condensation, and cell division. Histone H3 phosphorylation may initiate at different phases of the cell division in different organisms, but metaphase chromosomes are always found to be heavily phosphorylated. H3 phosphorylation at serine 28 is coupled with mitotic chromosome condensation in diverse mammalian cell lines. |
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| Catalog # | H5110-12M2 |
| Positive Control | anti-Fas mAb treated Jurkat |
| Applications | Suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilution | Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 8L222 |
| Host | Mouse |
| Source | Human |
| Concentration | As reported |
| Form | Containing 0.2% gelatin, 0.05% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | A synthetic phosphopeptide around serine at position 28 [BLAST]CARKS*APATGGVKK[/BLAST] of human Histone H3 was used as immunogen. The immunogen sequence (CARKS*APATGGVKK) is conserved across mammalian and invertebrates, as well as plants. |
| Specificity | Recognizes Histone H3 when phosphorylated at Ser28. In anti-Fas mAb treated Jurkat, an 18kD band is observed. The antibody has been evaluated in human and is predicted to work accross mutliple species due to conservation of the sequence used for immunogen. Species Crossreactivity: Human |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | H3, HisH3, His1H3, H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l |
