Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3 (Histone H3.3, H3 Histone Family 3A, H3F3A, H3F3B) (HRP)

Anti -Histone H3 (Histone H3.3, H3 Histone Family 3A, H3F3A, H3F3B) (HRP)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Rabbit Purified B
Modulation of chromatin structure plays an important role in the regulation of transcription in eukary-otes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The amino-
terminal tails of core histones undergo various post-transla-tional modifications, including acetylation, phosphorylation, methylation and ubiquitination (2-5). These modifications
occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15 and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18 and 23. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mam-
malian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase (11).
Catalog #H5110-12M5
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No10E27
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as liquid in PBS, pH 7.4, 2mg/ml BSA, 50% glycerol, 50% glycerol. Labeled with HRP.
PurityPurified
ImmunogenSynthetic peptide corresponding to the carboxy-terminal residues of human histone H3 (KLH).
SpecificityRecongnizes endogenous levels of human total histone H3 protein. Species crossreactivity: mouse, rat, monkey, pig, hamster, bovine, zebrafish.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links