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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3

Anti -Histone H3

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit B IC IF
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15 and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18 and 23. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase (11).
Catalog #H5110-12M8
ApplicationsSuitable for use in Western Blot, Immunohistochemistry (P) and Immunofluorescence (IF-IC). Other applications not tested.
Recommended DilutionWestern Blot: 1:2000 Incubate membrane with diluted antibody in 5% w/v nonfat dry
milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunohistochemistry (P): 1:400 Unmasking buffer: Citrate
Immunofluorescence (IF-IC):1:200 IF Protocol: Methanol Permeabilization required.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No10E29
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied in liquid 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and 0.02% sodium azide.
ImmunogenSynthetic peptide derived from the carboxy terminus of the human histone H3 protein (KLH-coupled).
SpecificityRecognizes human Acetyl-Histone H3 (Lys27). Species crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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