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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

The relatively unstructured and highly charged N-terminal tail domains of histones are central to the processes that modulate chromatin structure. A diverse and elaborate array of post-translational modifications, including acetylation, phosphorylation, and methylation occurs on the N-terminal tail domains of histones, particularly of H3 and H4.1, 2 These modifications may alter chromatin structure and recruit downstream chromatin-associated proteins involved in transcription regulation. These in turn may dictate dynamic transitions between transcriptionally active or silent chromatin states.
Histones H3 and H4 are the predominant histones modified by methylation and are highly methylated in mammalian cells.3, 4 Histone methylation, like acetylation, is a complex, dynamic process involving a number of processes, including transcriptional regulation, chromatin condensation, mitosis, and heterochromatin assembly. Moreover, lysine residues can be mono-, di-, and tri-methylated, adding further complexity to the regulation of chromatin structure.
Conserved lysine residues in the N-terminal tail domains of histone H3, Lys4, Lys9 and Lys27 are the preferred sites of methylation.1, 4-6 Methylation of H3 at Lys9 is a modification intrinsically linked to epigenetic silencing and heterochromatin assembly. Histone H3 is methylated at Lys9 by site-specific H3 methyltransferases (HMTases) encoded by the SUV39H1 gene family.7 Methylation of H3 at Lys9 by SUV39H1 generates a binding site for HP1 proteins, a family of heterochromatic adaptor proteins implicated in both gene silencing and in the organization of higher order chromatin.8-11 Methylation of Lys9 interferes with the phosphorylation of Ser10 but is also influenced by preexisting modifications in the N-terminus of H3, such as H3 Ser10 phosphorylation itself.7 Conversely, in vivo deregulated SUV39H1 or disrupted SUV39H1activity modulates H3 Ser10 phosphorylation in native chromatin leading to aberrant mitotic divisions.
Catalog #	H5110-12R1
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Concentration	~0.1mg/ml
Form	0.01 PBS, pH 7.4 containing 1.0% BSA
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide corresponding to amino acids 7-20 of Histone H3 conjugated to KLH.
Specificity	Detects a band at approximately 17kD. This Histone H3 sequences is identical in many species including mouse, rat, bovine, chicken, frog, drosophila and C. elegans. There is no inhibition with the non-methylated Histone H3 peptide. Species Crossreactivity: Crossreacts with Human. Expected to crossreact with mouse, rat, bovine, chicken, frog, drosophila and C. elegans.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	H3, HisH3, His1H3, H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l