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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3

Anti -Histone H3

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Specifications

Clone Host Grade Applications
Polyclonal Sheep E B IP IF
In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers.
Catalog #H5110-12R7
Cellular Localization Nuclear
Positive Control Tonsil, colon carcinoma and any other human tissues.
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceBovine
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2.
PurityAs reported
ImmunogenHistone 3, calf whole protein complexed to ribosomal RNA.
SpecificityRecognizes Histone H3
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesH3, HisH3, His1H3, H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l


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