| Autoantibodies to histone antigens have been described in patients with idiopathic and drug-induced SLE, rheumatoid arthritis, and other conditions. The presence of autoantibodies to histones are frequently found in several rheumatic disorders (1). In one study, the predominant responses to histones in SLE sera were to H1, H2b, and H3. Marked elevations of binding occurred to H1 and H2b in 33% of patients, while 25% showed higher binding to H3 (2). The same study showed the highest anti-histone reactivity to be in patients with rheumatoid arthritis with vasculitis, while the highest reactivity in SLE sera was in those patients with a history of photosensitivity (3). In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. |
|
| Catalog # | H5110-13 |
| Applications | |
| Suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 0.5-2.0ug/ml detects 17kD Histone H3 in acid extracted lysates from HeLa cells. Acid-extracted proteins from HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-13 (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat-anti rabbit and a chemiluminescence detection system. |
| Recommended secondary antibodies | I1905-28 IgM (HRP) Pab GtxMo |
| I1905-28A IgM (X-Adsorbed) (HRP) Pab GtxMo |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 5H2 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-glycine, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa1-20, ARTKQTARKSTGGK APRKQLC of human histone H3 (KLH). |
| Specificity | Recognizes and is specific for human histone H3 at ~17kD. Expected to crossreact with other species due to identity of the immunizing peptide across a wide range of species. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
