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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum B IP

Catalog #	H5110-13A
Applications	Suitable for use in Western Blot, Chromatin IP and Beadlyte Histone-Peptide Specificity Assay. Other applications not tested.
Recommended Dilution	Immunoblot Analysis: A 1:5000-1:10,000 dilution of this lot detected acetyl-Histone H3 (Lys9) in acid extracts from sodium butyrate treated HeLa cells.
	Chromatin Immunoprecipitation (ChIP): An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from a yeast strain containing a Lysine substitution to Alanine at residue 9.
	Beadlyte Histone-Peptide Specificity Assay: A 1:1000-1:5000 dilution of this lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres. No crossreactivity with peptides containing acetyl-lysine 14 or acetyl-lysine 27 was observed.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	Not determined
Form	Supplied as a liquid containing 0.05% sodium azide before the addition of glycerol to 30%.
Purity	Serum
Immunogen	Ovalbumin-conjugated, synthetic peptide (KQTARAcKSTGGK-C) corresponding to aa4-14 of yeast histone H3 acetylated on lysine 9, with a C-terminal cysteine added for conjugation purposes.
Specificity	Recognizes acetyl-histone H3 (Lys9), Mr 17kD. An additional unknown protein was detected at Mr 23kD. Species Crossreactivity: Yeast and human. Broad species crossreactivity is expected.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.