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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, acetylated (Lys56) (H3, H3/A, H3FA, Histone Cluster 1 H3a, HIST1H3A, Histone H3.1, Histone H3.3)

Anti -Histone H3, acetylated (Lys56) (H3, H3/A, H3FA, Histone Cluster 1 H3a, HIST1H3A,
Histone H3.1, Histone H3.3)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IH IC
Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr 3 in prophase and its dephosphorylation during anaphase. Furthermore we find that haspin, a member of a distinctive group of protein kinases present in diverse eukaryotes, phosphorylates H3 at Thr 3 in vitro. (5).
Catalog #H5110-13A5
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:2000
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
ptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9H379
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
PuritySupernatant.
ImmunogenSynthetic acetylated peptide corresponding to residues surrounding Lys 56 of Histone H3.
SpecificityRecognizes human Histone H3 acetylated on Lysine 56. Species Crossreactivity: Mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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