Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3

Anti -Histone H3

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B IP
Catalog #H5110-13C
In diploid eucaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and the basic proteins call Histones. The recurring positive charges of the histones form electrostataic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. Molecular weight 15.3kD.
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostSheep
SourceBovine
Concentration~2mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No preservative added.
PurityPurified by Protein G affinity chromatography.
ImmunogenIntact calf H-III complex to rRNA.
SpecificityRecognizes bovine Histone H3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links