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Specifications

Clone Host Grade Applications Polyclonal Sheep Affinity Purified B IP

Catalog #	H5110-13C
In diploid eucaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and the basic proteins call Histones. The recurring positive charges of the histones form electrostataic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. Molecular weight	15.3kD.
Applications	Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Sheep
Source	Bovine
Concentration	~2mg/ml
Form	Supplied as a liquid in PBS, pH 7.4. No preservative added.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Intact calf H-III complex to rRNA.
Specificity	Recognizes bovine Histone H3.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.