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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH IC IF

Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation (2–4). These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20 (4,5). Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23 (2,3). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (6).
Catalog #	H5110-13DX
Applications	Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.
Recommended Dilution	Western Blot: 1:1000
	Immunohistochemistry (Paraffin): 1:25
	Immunocytochemistry (IF): 1:50
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	~0.25mg/ml
Form	Supplied as liquid in PBS. Also available with BSA & Glycerol. See H5110-13D.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Synthetic peptide (KLH coupled) derived from the C-terminal sequence of human histone H3.
Specificity	Detects total levels of endogenous histone H3 protein. This antibody does not recognize other histones. Note: Adherence to the attached protocol is extremely important.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.