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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, acetylated (Lys9)

Anti -Histone H3, acetylated (Lys9)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH IC
Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different post-translational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis.
Catalog #H5110-13G
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry (Paraffin sections) and Immunocytochemistry (ABC detection method). Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:100
Immunocytochemistry (ABC): 1:500
Optimal dilutions to be determined by the researcher.
Important Note Adherence to the attached protocol is extremely important.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A and peptide affinity chromatography.
ImmunogenSynthetic acetylated peptide corresponding to residues surrounding Lys9 of histone H3.
SpecificityRecognizes endogenous levels of human Histone H3 only when acetylated at lysine 9 at 17kD. It does not crossreact with phosphorylated histone H3. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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