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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, acetylated (Agarose)

Anti -Histone H3, acetylated (Agarose)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IP
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysines in histone H3 is associated with genes that are being actively transcribed into RNA.
Catalog #H5110-13H5
ApplicationsSuitable for use in Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunoprecipitation: 20ul of a 50% slurry.
Optimal dilutions to be determined by the researcher.
Positive ControlTSA-treated or sodium butyrate-treated HeLa or NIH/3T3 cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceTetrahymena
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.05% sodium azide. Conjugated to Agarose.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to tetrahymena Acetyl-Histone H3. Cellular Localization
SpecificityRecognizes Tetrahymena Acetylated histone H3. Species Crossreactivity: human, mouse. Due to immunogen conservation, wide species reactivity is expected.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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