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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified IP

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysines in histone H3 is associated with genes that are being actively transcribed into RNA.
Catalog #	H5110-13H5
Applications	Suitable for use in Immunoprecipitation. Other applications not tested.
Recommended Dilution	Immunoprecipitation: 20ul of a 50% slurry.
	Optimal dilutions to be determined by the researcher.
Positive Control	TSA-treated or sodium butyrate-treated HeLa or NIH/3T3 cells.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Tetrahymena
Concentration	As reported
Form	Supplied as a liquid in PBS, 0.05% sodium azide. Conjugated to Agarose.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Synthetic peptide corresponding to tetrahymena Acetyl-Histone H3. Cellular Localization
Specificity	Recognizes Tetrahymena Acetylated histone H3. Species Crossreactivity: human, mouse. Due to immunogen conservation, wide species reactivity is expected.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.