Anti -Histone H3, phosphorylated (Ser10)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Ascites |
E B IC FC IF |
|
| Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation (2–4). These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20 (4,5). Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23 (2,3). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (6). | | | Catalog # | H5110-13K | | Applications | Suitable for use in ELISA, Western Blot,, Immunofluorescence, Immunocytochemistry and Flow Cytometry. Other applications not tested. | | Recommended Dilution | Immunofluorescence(IF-IC): 1:100 | | Immunofluorescence(IF-F): 1:100 | | Flow Cytometry: 1:25 | | Western Blot: 1:1000 Incubate membrane with diluted antibody in 1X TBS, 0.1% Tween-20, 5% BSA at 4°C with gentle shaking, overnight. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 3A50 | | Host | Mouse | | Source | Human | | Concentration | Not Determined | | Form | Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. | | Purity | Ascites. | | Immunogen | Synthetic phosphopeptide corresponding to residues around Ser10 of human histone H3 (KLH). | | Specificity | Recognizes human histone H3 only when phosphorylated at Ser10 at 17kD. Does not cross-react with other phosphorylatied histones or acetylated of histone H3. Species Cross-reactivity: Mouse, rat, Drosophila. | | | Note | Adherence to the attached protocol is extremely important. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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