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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, acetylated (Lys27)

Anti -Histone H3, acetylated (Lys27)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP
Catalog #H5110-13W
ApplicationsSuitable for use in Western Blotting, Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot : 1:1000-1:10,000 detected acetyl-Histone H3 (Lys27) in acid extracts from sodium butyrate treated HeLa cells
Immunoprecipitation : Immunoprecipitates chromatin from wild type yeast, compared to yeast containing a Histone H3 K27R substitution.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
FormFrozen serum, 0.01% thimerosal.
PuritySerum
ImmunogenOvalbumin-conjugated, synthetic peptide (SKAARAcKSAPST-C) corresponding to amino acids 22-32 of yeast Histone H3 acetylated on lysine 27, with a C-terminal cysteine added for conjugation purposes.
SpecificityRecognizes acetyl-Histone H3 (Lys27), Mr ~17kD. Species Crossreactivity: Yeast and human. Broad species crossreactivity is expected.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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