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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, acetylated (Lys9)

Anti -Histone H3, acetylated (Lys9)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B Gel
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.
Catalog #H5110-14A
ApplicationsSuitable for use in Western and Dot Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1:500. Detects acetylated histone H3 on 10ug of HEK293 lysates.
Dot Blot: 0.5ug/ml. Detects 50ng of mono-acetylated peptide corresponding to position Lys9 in the N-terminal sequence of Histone H3. Does not detect the mono-acetylated peptide corresponding to acetyl-lysine at position 14 or unacetylated Histone H3.
Optimal dilutions to be determined by the researcher.
Positive ControlTSA-treated HeLa and NIH/3T3 cells.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa1-12, ARTKQTAR(AcK)STG-C, where (AcK) denotes acetyl-lysine of human Histone H3, conjugated to KLH (NP_003484). Peptide sequence is identical in a wide range of animal and plant species.
SpecificityRecognizes human Histone H3 (Mr 17kD) acetylated at position Lys 9. Species Crossreactivity: mouse, rat and Eukaryote.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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