Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, acetylated (Lys9)

Anti -Histone H3, acetylated (Lys9)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH FC IF
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4) is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6). In most species, histone H2B is primarily acetylated at lysines 5, 12, 15 and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18 and 23 (2,3). Acetylation at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Staining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase (11).
Catalog #H5110-14A1
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Chromatin Immunoprecipitation: 1:50
Immunoprecipitation: 1:25
Immunofluorescence (IF-IC): 1:400
Immunohistochemistry (paraffin): 1:800. Recommend antigen retrieval by heating in citrate buffer.
Flow Cytometry: 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No7H26
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.15M sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to the amino terminus of histone H3 in which Lys9 is acetylated.
SpecificityRecognizes endogenous levels of human histone H3 at ~17kD only when acetylated on Lys9. Does not crossreact with other acetylated histones. Species Crossreactivity: mouse, rat, monkey, zebrafish, C. elegans and S. cerevisiae.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links