| Catalog # | H5110-14B |
| Applications | Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested. |
| Recommended Dilutions | Western Blot: 0.5-1ug/ml detected histone H3on 10ug of Colemid treated HeLa acid extract. Colemid treated HeLa acid was resolved by electrophoresis, transferred to PVDF and probed with H5110-14B. Proteins were visualized using a goat anti-rabbit secondary antibody (HRP) and a chemiluminescence detection system. |
| Immunoprecipitation: 10ug specifically immunoprecipitated phosphorylated histone H3 from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate. |
| Immunocytochemistry: 1:500 detected Histone H3 in HeLa and A431 cells. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-47H: IgG, H&L (HRP) Pab GtxRt |
| I1904-47N: IgG, H&L, X-Adsorbed (HRP) Pab GtxRt |
| I1904-47T: IgG, H&L (HRP) Pab GtxRt |
| I1904-50T1: IgG, H&L (HRP) Pab GtxRt |
| I1904-50U1: IgG H&L, X-adsorbed (HRP) Pab GtxRt |
| I1904-40D: IgG, H&L (FITC) Pab Gt xRb |
| I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb |
| I1904-46N: IgG, H&L (FITC) Pab Gt xRb |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Linear peptide coresponding to human Histone H3 at Ser10 |
| Specificity | Recognizes human histone H3 phosphorylated at Serine 10 at ~17kD. Species Crossreactivity: mouse. Broad species reactivity expected due to sequence homology. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
