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You are here:Home » Antibodies » Antibodies-Histones » Anti -Histone H3, phosphorylated (Ser10) (Mitosis Marker)

Anti -Histone H3, phosphorylated (Ser10) (Mitosis Marker)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IC
Catalog #H5110-14B
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 0.5-1ug/ml detected histone H3on 10ug of Colemid treated HeLa acid extract. Colemid treated HeLa acid was resolved by electrophoresis, transferred to PVDF and probed with H5110-14B. Proteins were visualized using a goat anti-rabbit secondary antibody (HRP) and a chemiluminescence detection system.
Immunoprecipitation: 10ug specifically immunoprecipitated phosphorylated histone H3 from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate.
Immunocytochemistry: 1:500 detected Histone H3 in HeLa and A431 cells.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-47H: IgG, H&L (HRP) Pab GtxRt
I1904-47N: IgG, H&L, X-Adsorbed (HRP) Pab GtxRt
I1904-47T: IgG, H&L (HRP) Pab GtxRt
I1904-50T1: IgG, H&L (HRP) Pab GtxRt
I1904-50U1: IgG H&L, X-adsorbed (HRP) Pab GtxRt
I1904-40D: IgG, H&L (FITC) Pab Gt xRb
I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb
I1904-46N: IgG, H&L (FITC) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenLinear peptide coresponding to human Histone H3 at Ser10
SpecificityRecognizes human histone H3 phosphorylated at Serine 10 at ~17kD. Species Crossreactivity: mouse. Broad species reactivity expected due to sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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