Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested.

Western Blot: 0.5-1ug/ml detected histone H3 on 10ug of Colemid treated HeLa acid extract. Colemid treated HeLa acid was resolved by electrophoresis, transferred to PVDF and probed with H5110-14B. Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system. Immunoprecipitation: 10ug specifically immunoprecipitated phosphorylated histone H3 from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate. Immunocytochemistry: 1:500 detected Histone H3 in HeLa and A431 cells. Optimal dilutions to be determined by the researcher.

I1904-47H: IgG, H&L (HRP) Pab GtxRt I1904-47N: IgG, H&L, X-Adsorbed (HRP) Pab GtxRt I1904-47T: IgG, H&L (HRP) Pab GtxRt I1904-50T1: IgG, H&L (HRP) Pab GtxRt I1904-50U1: IgG H&L, X-adsorbed (HRP) Pab GtxRt I1904-40D: IgG, H&L (FITC) Pab Gt xRb I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb I1904-46N: IgG, H&L (FITC) Pab Gt xRb

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after recei. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Linear peptide coresponding to human Histone H3 at Ser10

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

Purified by Protein A affinity chromatography.

Recognizes human histone H3 phosphorylated at Serine 10 at ~17kD. Species Crossreactivity: mouse. Broad species reactivity expected due to sequence homology.