Anti -Histone H3 (H3F3A, 601128, Q66I33, H3.3A, H3F3, MGC87782, MGC87783, 3021, H3F3B, 601058, P84243, H3.3B, H3F3A)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IH |
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| The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. | | | Catalog # | H5110-14C2 | | Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. | | Recommended Dilutions | | | Western Blot: 0.5-4ug/ml | | Immunohistochemistry: 20ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol. | | Purity | Purified by Immunoaffinity chromatography. | | Immunogen | Synthetic peptide near the C-terminus of human Histone H3 (Internal ID# BV-B14). | | | Specificity | Recognizes human Histone H3 at ~17-20 kD. Does not cross-react with other histones. Species crossreactivity: mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Histone H3 (H3F3A, 601128, Q66I33, H3.3A, H3F3, MGC87782, MGC87783, 3021, H3F3B, 601058, P84243, H3.3B, H3F3A) |
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