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You are here:Home » Antibodies » Antibodies-Histones » Anti -Histone H3 (H3F3A, 601128, Q66I33, H3.3A, H3F3, MGC87782, MGC87783, 3021, H3F3B, 601058, P84243, H3.3B, H3F3A)

Anti -Histone H3 (H3F3A, 601128, Q66I33, H3.3A, H3F3, MGC87782, MGC87783, 3021, H3F3B,
601058, P84243, H3.3B, H3F3A)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.
Catalog #H5110-14C2
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 0.5-4ug/ml
Immunohistochemistry: 20ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
PurityPurified by Immunoaffinity chromatography.
ImmunogenSynthetic peptide near the C-terminus of human Histone H3 (Internal ID# BV-B14).
SpecificityRecognizes human Histone H3 at ~17-20 kD. Does not cross-react with other histones. Species crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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