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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, dimethyl (Lys9)

Anti -Histone H3, dimethyl (Lys9)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit B IP IF
The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1) and WD-40 domains (WDR5) (5-8). The recent discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2 and JHDM1 has shown that methylation is a reversible epigenetic mark (9).
Catalog #H5110-14D2
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunofluorescence (IF-IC) and Immunoprecipitation (Chromatin). Other applications not tested.
Recommended DilutionWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Immunofluorescence (IF-IC) 1:200
Chromatin Immunoprecipitation (CHIP): 1:25
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No10E30
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied in liquid 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and 0.02% sodium azide.
ImmunogenSynthetic peptide corresponding to the amino terminus of histone H3 in which Lys9 is di-methylated (KLH- coupled).
SpecificityRecognizes human Di-Methyl-Histone H3 (Lys9) (D85B4). Species crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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