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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Catalog #	H5110-14E
Applications	Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilution	Western Blot: 1:500-1:5000 detects dimethyl Histone H3 on 10ug of HeLa acid extract but not on recombinant Histone H3. HeLa acid extract were resolved by electrophoresis, transferred to PVDF membrane and probed with H5110-14E (1:500). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
	Optimal dilutions to be determined by the researcher.
Positive Control	HeLa, NIH/3T3 or COS cell extracts
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M sodium chloride, 5mg/ml BSA, 0.05% sodium azide, 30% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide corresponding to aa6-13, TAR[dimethyl-K]STGG-C of human Histone H3 with a C added to C-terminus (KLH). The immunizing sequence is conserved from Tetrahymena to human.
Specificity	Recognizes methylated human Histone H3 (Lys9) at ~17kD. Slight crossreactivity to the trimethyl Lys27 Histone H3. Species Crossreactivity: Fission yeast and chicken.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.