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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant E B

Catalog #	H5110-14E1
Applications	Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution	Immunoblot Analysis: 1:500-1:5000 of this lot detected dimethyl (Lys9) histone H3 in acid extracted proteins from HeLa cells and core histones purified from chicken erythrocytes, recombinant Xenopus Histone H3 methylated in vitro by G9a methyltransferase but not unmethylated recombinant Histone H3.
	ELISA: 1:300-1:2700 of this lot detected the immunizing peptide but not a corresponding unmethylated peptide. No crossreactivity detected with Histone H3 derived peptides containing dimethyl-lysine at residues corresponding to 4, 14, 27, 36 or 79.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM
Clone No	5H11
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid containing 0.05% sodium azide and 30% glycerol.
Purity	Culture supernatant
Immunogen	KLH-conjugated, synthetic peptide containing ..AR[dimethyl-K]ST.. in which dimethyl-K corresponds to dimethyl-lysine at residue 9 of human
histone H3. The immunizing sequence is conserved from Tetrahymena to human.
Specificity	Recognizes dimethylated Histone H3 (Lys9), Mr ~17kD. Specificity demonstrated by ELISA using methylated and unmethylated peptides. Species Crossreactivity: Human, Chicken and Xenopus.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.