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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, Dimethyl (Lys9)

Anti -Histone H3, Dimethyl (Lys9)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B
Catalog #H5110-14E3
ApplicationsSuitable for use in Western Blot, Peptide Inhibition and Beadlyte® assay. Other applications not tested.
Recommended DilutionImmunoblot Analysis: A 1:1000-1:5000 dilution of this lot detected methylated Histone H3 in acid-extracted proteins from HeLa cells. The antibody did not detect unmethylated recombinant Histone H3.
Peptide Inhibition: Specificity of this lot was confirmed by the ability of 1uM of the immunizing peptide to completely abolish detection of Histone H3 in immunoblot analysis of HeLa acid extracts. No competition was observed with peptides containing monomethyl-lysine 9, trimethyl-lysine 9, monomethyl-lysine 27, dimethyl-lysine 27, or trimethyl-lysine 27.
Beadlyte® Histone-Peptide Specificity Assay: A 1:1000 dilution of this lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex microspheres.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid containing 0.05% and 30% glycerol.
PuritySerum depleted of non-specific Histone H3 antibodies
ImmunogenKLH-conjugated synthetic peptide containing the sequence ..ARdmKS… in which dmK corresponds to dimethyl-lysine at residue 9 of human
histone H3. The immunizing sequence is conserved from Tetrahymena to human.
SpecificityRecognizes dimethylated Histone H3 (Lys9), Mr 17kD with a nonspecific protein detected at 97kD. Species Crossreactivity: Human. A broad species crossreactivity is expected.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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