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Anti -Histone H3, Dimethyl (Lys9) (Biotin)
Anti -Histone H3, Dimethyl (Lys9) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| Catalog # | H5110-14F | | Applications | Suitable for use in Immunoblot. Other applications not tested. | | Recommended Dilutions | Immunoblot: A 1:1000. Detects methylated Histone H3 in acid-extracted proteins from HeLa cells. The antibody did not detect recombinant histone H3 puri ed from E. coli. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide. Labeled with Biotin. Biotin | | Purity | Purified by affinity chromatography. | | Immunogen | Synthetic peptide corresponding to aa6-13 (TAR[dimethyl-K] STGG-C) of Histone H3(C-terminal cysteine added to facilitate KLH coupling). The immunizing sequence is conserved from Tetrahymena to human. | | Specificity | Recognizes methylated Histone H3 (Lys9), Mr 17kD. Speci city demonstrated by ELISA using methylated and unmethylated peptides. Does not recognize recombinant histone H3 puri ed from E. coli. Species Crossreactivity: Human, ssion yeast and chicken. Broad species crossreactivity is expected. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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