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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, Dimethyl (Lys9) (Biotin)

Anti -Histone H3, Dimethyl (Lys9) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #H5110-14F
ApplicationsSuitable for use in Immunoblot. Other applications not tested.
Recommended DilutionsImmunoblot: A 1:1000. Detects methylated Histone H3 in acid-extracted proteins from HeLa cells. The antibody did not detect recombinant histone H3 puri ed from E. coli.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide. Labeled with Biotin. Biotin
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa6-13 (TAR[dimethyl-K] STGG-C) of Histone H3(C-terminal cysteine added to facilitate KLH coupling). The immunizing sequence is conserved from Tetrahymena to human.
SpecificityRecognizes methylated Histone H3 (Lys9), Mr 17kD. Speci city demonstrated by ELISA using methylated and unmethylated peptides. Does not recognize recombinant histone H3 puri ed from E. coli. Species Crossreactivity: Human, ssion yeast and chicken. Broad species crossreactivity is expected.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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