| Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. |
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| Catalog # | H5110-14G |
| Applications | Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested. |
| Recommended Dilutions | ELISA: 1:2000 is specific for peptides containing dimethylated Arg17. No crossreactivity detected with a peptide containing dimethylated Arg26. |
| Western Blot: 1:1000-1:2000 detected in-vitro methylated Histone H3. Products from an in vitro methyl transferase reaction were resolved by electrophoresis and probed with H5110-14G. Proteins were visualized using an IgG (HRP) Gt xRb* and a chemiluminescence detection system. |
| Immunocytochemistry: Reported to show positive immunostaining for CARM-1 speci c methylation of Histone H3 in 3134 cells. |
| Optimal dilutions to be determined by the researcher. |
| *Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb |
| Recommended Control | Celf thymus histone preparation, Acid HeLa cell extract |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in 0.14M Tris-glycine, pH 7.4, 0.105M sodium chloride, 3.5mg/ml BSA, 0.035% sodium azide, 30% glycerol. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa11-21, TGGKAPMe2RKQLA-C of human Histone H3 (BSA). |
| Specificity | Recognizes human methylated Histone H3, Mr 17kD. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
