Anti -Histone H3, acetylated (Lys9) (H3, H3/A, H3FA, Histone Cluster 1 H3a, HIST1H3A,
Histone H3.1, Histone H3.3)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP IH IC |
|
| Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. | | | Catalog # | H5110-14H9 | | Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:500 | | Immunohistochemistry (paraffin): 1:50-100 | | Immunocytochemistry: 1:250 | | Immunoprecipitation: 1:40 | | Optimal dilutions to be determined by the researcher. | | Positive Control | Untreated and TSA-treated NIH/3T3 cell lysate. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 9i169 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol. | | Purity | Supernatant | | Immunogen | Acetylated-synthetic peptide corresponding to residues surrounding Lys9 of Histone H3. Cellular Localization: Nucleus. | | Specificity | Recognizes human Histone H3, acetylated on Lys9. Species Crossreactivity: mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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