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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, Dimethyl (Lys4)

Anti -Histone H3, Dimethyl (Lys4)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IP IC
Catalog #H5110-14J
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunocytyochemistry. Other applications not tested.
Recommended DilutionsWestern Blot Analysis: 1:2000-1:10,000 detects methylated Histone H3 in acid extracted proteins from HeLa cells. 1:20001:5000 dilution of a previous lot detected methylated Histone H3 in acid extracted proteins from HeLa and tetrahymena cells. HeLa cell acid precipitate was resolved by electrophoresis, transferred to nitrocellulose and probed with a 1:5000 dilution of H5110-14J. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Chromatin Immunoprecipitation: 5ul immunoprecipitates methylated Histone H3 from1x10e6 3T3/A31 cells.
Immunocytochemistry: 1:500 shows positive immunostaining for methylated Histone H3 in L6 cells xed with 4% paraformaldehyde and permeabilized using 0.1% Triton X-100.
Optimcal dilutions should be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
I1904-40D: IgG, H&L (FITC) Pab Gt xRb
I1904-40M: IgG, Fc, X-Adsorbed (FITC) Pab Gt xRb
I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb
I1904-46N: IgG, H&L (FITC) Pab Gt xRb
Positive ControlHeLa whole cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as liquid, 0.05% sodium azide, 30% glycerol.
PuritySerum
ImmunogenSynthetic peptide corresponding to human Histone H3 (aa1-8, ART(Me2)KQTAR-GC, N-terminal) (KLH).
SpecificityRecognizes human dimethylated Histone H3 (Lys4), Mr 17kD. Speci city demonstrated by ELISA using methylated peptides.
Species CrossreactivityTetrahymena.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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