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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, phosphorylated (Ser10) (Biotin)

Anti -Histone H3, phosphorylated (Ser10) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IC
Catalog #H5110-14K
ApplicationsSuitable for use in Immunocytochemistry. Other applications not tested.
Recommended DilutionsImmunocytochemistry: 2-5ug/ml. Positive immunostaining of chromatin in HeLa cells undergoing mitosis. Cells were xed with 4% paraformaldehyde and permeablized with 0.25% TritonX.
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide. Labeled with Biotin.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa7-20 (ARK[pS]TG-GKAPRKQL- C) of human histone H3.
SpecificityRecognizes and is speci c for phosphorylated histone H3 (~17kD). Species Crossreactivity: Human. Broad species crossreactivity expected.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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