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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, dimethyl (Lys4, Lys9)

Anti -Histone H3, dimethyl (Lys4, Lys9)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IC
Catalog #H5110-14Q
ApplicationsSuitable for use in Western Blotting, Immunoprecipitation, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot : 1:5,000 detects dimethyl (Lys4) dimethyl (Lys9) histone H3 in acid
extracted proteins from HeLa cells and core histones purified from chicken erythrocytes, but not unmethylated recombinant Histone H3.
Immunocytochemistry 1:200. Positive immunostaining for dimethyl (Lys4) dimethyl (Lys9) histone H3 in HeLa cells fixed with 3.7% formaldehyde.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
FormSupplied as a liquid in .5M sodium borate, pH 8.2, 0.15M sodium chloride, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 1-12 acids (ART(dimethyl-K)ATAR(dimethyl-K)STG-C) of human
histone H3 with a C-terminal cysteine added for conjugation purposes.
SpecificityRecognizes dimethyl (Lys4) dimethyl (Lys9) histone H3, Mr ~17kD. Specificity
demonstrated by dot blot using methylated peptides Species CrossreactivityHuman and chicken. Broad species crossreactivity is expected.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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