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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, dimethyl (Lys4, Lys9) (H3 histone, family 3A, Histone H3.3, H3F3A, H3.3A, H3F3, H3F3B, H3.3B)

Anti -Histone H3, dimethyl (Lys4, Lys9) (H3 histone, family 3A, Histone H3.3, H3F3A,
H3.3A, H3F3, H3F3B, H3.3B)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.HeLa nuclear extract
Catalog #H5110-14Q1
ApplicationsSuitable for use in Western Blot, Multiplexing, and Chromatin Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to residues surrounding and including dimethlyated Lys 4 and dimethlyated Lys9 of Histone H3. Epitope: Dimethylated Lys4 & Lys9
SpecificityRecognizes human Histone H3 only when dimethylated on both Lysine 4 and Lys9. Expected to react with most other species based on extreme conserved homology among species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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