| The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (1). |
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| Catalog # | H5110-14S3 |
| Applications | Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Immunofluorescence (IC): 1:400 |
| Western Blot: 1:1000 |
| Immunoprecipitation: 1:50 |
| Chromatin Immunoprecipitation: 1:25 |
| Immunohistochemistry (paraffin): 1:100 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Concentration | Not determined |
| Form | Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. |
| Purity | Purified by Immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys27 is tri-methylated (KLH). Species Sequence Homology: Xenopus (100%). |
| Specificity | Recognizes endogenous levels of human histone H3 only when tri-methylated on Lys27. Does not crossreact with non-methylated, mono-methylated or di-methylated Lys27. In addition, the antibody does not crossreact with mono-methylated, di-methylated or tri-methylated histone H3 at Lys4, Lys9, Lys36 or Histone H4 at Lys20. Species Crossreactivity: human, mouse, rat and monkey. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
