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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IF

The methylation of specific histone lysines controls aspects of short- and long-term transcriptional regulation, in addition to the propagation of bulk chromosome structure and stability. Methylated histone H3 K27 is also involved in transcriptional silencing of human polycomb target genes and earliest chromatin changes in the X-chromosome inactivation. Methylated histone H3 K27 is considered a mark of repressive chromatin states.
Catalog #	H5110-14S6
Applications	Suitable for use in Immunofluorescence and ELISA. Other applications not tested.
Recommended Dilution	Immunofluorescence: 1:1000
	ELISA: 1:1000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG3
Clone No	9F617
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2.
Purity	Purified by Protein G affinity chromatography.
Immunogen	KLH-conjugated synthetic peptide TKAAR[dimethyl-K]SAP-C corresponding to aa22-30 of human histone H3
Specificity	Recognizes human di- and trimethylated H3 K27
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.