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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, dimethyl

Anti -Histone H3, dimethyl

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP
Catalog #H5110-14T
ApplicationsSuitable for use in Western Blotting, Chromatin Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot : 1:2,000 to 1:10,000 detected dimethyl-Histone H3 (Lys36) in acid extracts from HeLa cells but not unmethylated recombinant Histone H3
Chromatin Immunoprecipitation: immunoprecipitates formalincross-linked chromatin containing Histone H3 methylated at lysine residue 36.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
FormFrozen serum, 0.05% sodium azide.
PuritySerum
ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 31-41 (STGGV[dimethyl-K]KPHRY-C) of human, mouse, rat, and yeast histone H3 dimethylated on lysine 36, with a C-terminal cysteine added for conjugation purposes.
SpecificityRecognizes dimethyl-Histone H3 (Lys36), Mr ~17kD. Species Crossreactivity: Broad, based on sequence conservation.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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