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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, dimethyl

Anti -Histone H3, dimethyl


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Clone Host Grade Applications
Polyclonal Rabbit Serum B IP
Catalog #H5110-14T
ApplicationsSuitable for use in Western Blotting, Chromatin Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot : 1:2,000 to 1:10,000 detected dimethyl-Histone H3 (Lys36) in acid extracts from HeLa cells but not unmethylated recombinant Histone H3
Chromatin Immunoprecipitation: immunoprecipitates formalincross-linked chromatin containing Histone H3 methylated at lysine residue 36.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormFrozen serum, 0.05% sodium azide.
ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 31-41 (STGGV[dimethyl-K]KPHRY-C) of human, mouse, rat, and yeast histone H3 dimethylated on lysine 36, with a C-terminal cysteine added for conjugation purposes.
SpecificityRecognizes dimethyl-Histone H3 (Lys36), Mr ~17kD. Species Crossreactivity: Broad, based on sequence conservation.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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