| Catalog # | H5110-14V |
| Applications | Suitable for use in Western Blot, Peptide Inhibition and Peptide Dot Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:2,000-1:12,000 detects dimethyl-histone H3 (Arg2) in acid-extracted proteins from HeLa cells.. Acid extracts from HeLa cells or recombinant Histone H3 were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-dimethyl-Histone H3 (Arg2) (1:6,000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. |
| Peptide Inhibition | 2uM of histone H3 peptide containing dimethyl-arginine 2 abolished |
| detection of histone H3 by anti-dimethyl-Histone H3 (Arg2) (1:4,000 dilution) in immunoblots of HeLa acid extracts. Some competition of signal was also seen with unmodified peptide containing arginine 2. HeLa acid extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-dimethyl-Histone H3 (Arg2) (1:4,000, Lane 1), or with the antibody pre-absorbed with 2uM histone H3 peptides. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. |
| Peptide Dot Blot | 1:4,000-1:16,000 detected histone H3 peptide containing dimethyl-arginine 2. Peptides containing unmodified arginine 2, dimethyl-arginine17 or dimethyl-arginine 26 were not detected. A dilution series of histone H3 peptides. 2ul of each dilution was spotted onto a Hybond™-P nylon membrane and probed with anti-dimethyl-Histone H3 (Arg2) (1:4,000 dilution). Peptides were visualized using a goat anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 4H342 |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 100uM EDTA, 0.05% sodium azide, before the addition of glycerol to 30%. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | KLH-conjugated, synthetic peptide containing the sequence …Ame2RTKQ…, in which me2R corresponds to dimethyl-arginine 2 of human histone H3. |
| Specificity | Recognizes dimethyl-arginine at residue 2 of histone H3, Mr 17kD. Species Crossreactivity: Human. Broad species crossreactivity is expected. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
