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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

Catalog #	H5110-14W
Applications	Suitable for use in Immunoblot. Other applications not tested.
Recommended Dilution	Western Blot: 1:500-1:2000 detected methylated Histone H3 in acid extracted proteins from HeLa cells, but did not detect unmethylated recombinant Histone H3. Recombinant Histone H3 and HeLa cell acid precipitate were resolved by electrophoresis, transferred to nitrocellulose and probed with a 1:500 dilution of anti-dimethyl Histone H3 (Lys14). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence
	detection system.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	As reported
Form	Supplied as a liquid in 50mM Tris, pH 7.4, 150mM NaCl, 0.05% sodium azide, before the addition of glycerol to 30%.
Purity	Purified by immunoaffinity chromatography.
Immunogen	KLH conjugated synthetic peptide (...GG[dimethyl-K]AP…), in which dimethyl-K corresponds to lysine residue 14 of human Histone H3.
Specificity	Recognizes dimethylated Histone H3 (Lys14), Mr ~17kD. Species Crossreactivity: Human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.