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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, dimethyl (Lys14)

Anti -Histone H3, dimethyl (Lys14)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #H5110-14W
ApplicationsSuitable for use in Immunoblot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:2000 detected methylated Histone H3 in acid extracted proteins from HeLa cells, but did not detect unmethylated recombinant Histone H3. Recombinant Histone H3 and HeLa cell acid precipitate were resolved by electrophoresis, transferred to nitrocellulose and probed with a 1:500 dilution of anti-dimethyl Histone H3 (Lys14). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence
detection system.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in 50mM Tris, pH 7.4, 150mM NaCl, 0.05% sodium azide, before the addition of glycerol to 30%.
PurityPurified by immunoaffinity chromatography.
ImmunogenKLH conjugated synthetic peptide (...GG[dimethyl-K]APů), in which dimethyl-K corresponds to lysine residue 14 of human Histone H3.
SpecificityRecognizes dimethylated Histone H3 (Lys14), Mr ~17kD. Species Crossreactivity: Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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