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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, dimethyl (Lys23)

Anti -Histone H3, dimethyl (Lys23)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #H5110-14W5
ApplicationsSuitable for use in Western Blot, Dot Blot and Beadlyte® Histone Peptide Assay. Other applications not tested.
Recommended DilutionImmunoblot Analysis: A 1:500-1:1000 dilution of this lot detected dimethylated Histone H3 (Lys23) in acid extracted proteins from HeLa cells.
Peptide Dot Blot Analysis: A 1:1000-1:2000 dilution of this lot only detected the peptide containing the dimethylated histone H3 (Lys23) modification.
Beadlyte® Histone Peptide Assay: 1:2000 to 1:54000 dilution of this lot only detected the peptide containing the dimethylated Histone H3 (Lys23) modification.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M sodium chloride and 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by immunoaffinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide containing the sequence… ATdmKAA…in which dmK corresponds to dimethylated lysine 23 of human histone H3
SpecificityRecognizes dimethyl Histone H3 (Lys23), Mr 17kD. Species Crossreactivity: Human. Broad species crossreactivity is expected.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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