| Histone H3 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). |
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| Catalog # | H5110-14Y |
| Applications | Suitable for use in Western Blot, Peptide Inhibition, Immunocytochemistry, Chromatin Immunoprecipitation, Beadlyte Histone-Peptide Specificity Assay and Dot Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 0.5ug/ml detects dimethylated histone H3 in acid extracted proteins from HeLa cells. No detection of 1ug unmethylated recombinant Histone H3 (H5110-12K) was observed. |
| Peptide Inhibition: 1uM of the immunizing peptide to completely abolishes detection of histone H3 in Western Blot analysis of HeLa acid extracts. Slight signal reduction was observed with peptide containing dimethyl-lysine 9. Signal was not reduced by branched monomethyl H3(Lys27), linear monomethyl H3(Lys27), linear trimethylH3(Lys27), or linear dimethyl H3(Lys4) peptides. |
| Beadlyte Histone-Peptide Specificity Assay: 12.5ng/ml or a range of dilutions detected peptides containing the dimethyl-lysine modification at residue 27. Peptides containing other modifications were not detected. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic branched peptide containing the sequence ARme2KSA… in which me2K corresponds to dimethyl-lysine at residue 27 of human histone H3 (KLH). |
| Specificity | Recognizes human dimethylated histone H3 (Lys27), Mr 17kD. At high concentrations, some crossreactivity with dimethyl histone H3 (Lys9) occurs. Broad species crossreactivity expected, based on sequence homology. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
