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Specifications

Clone Host Grade Applications Polyclonal Chicken Affinity Purified B IP

Catalog #	H5110-14Y2
Applications	Suitable for use in Western Blot and Chromatin IP. Other applications not tested.
Recommended Dilution	Immunoblot Analysis: 1:500-1:1000 dilution of this lot detected methylated Histone H3 in acid extracted proteins from HeLa cells, but did not detect unmethylated recombinant Histone H3. This may also be tested using Histone H3.
	Chromatin Immunoprecipitation: Reported by an independent laboratory to immunoprecipitate chromatin using an anti-chicken bridging antibody to mediate protein A binding.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgY
Host	Chicken
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Purity	Purified by immunoaffinity chromatography.
Immunogen	KLH-conjugated synthetic peptide (TKAAR[dimethyl-K]SAPST-C) corresponding to aa22-32 of human Histone H3, with a C-terminal cysteine added for conjugation purposes.
Specificity	Recognizes dimethylated Histone H3 (Lys27), Mr 17kD. Preference for dimethyl-Lys27 over monomethyl-Lys27, 27trimethyl-Lys27 and dimethyl-Lys9 verified by ELISA using corresponding methylated peptides. Species Crossreactivity: Human, mouse, rat, cow, fly and chicken. Does not react with yeast. Broad crossreactivity expected based on homology of Histone H3.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.