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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, dimethyl (Lys36)

Anti -Histone H3, dimethyl (Lys36)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Catalog #H5110-14Y6
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: A 1:1000-1:3000 detects methylated Histone H3 in acid-extracted proteins from untreated HeLa cells. Did not detect recombinant Xenopus laevis histone H3 purified from E. coli.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid, 0.05% sodium azide, before the addition of glycerol to 30%.
PurityImmunodepleted serum; Serum was passed over immunoaffinity column containing the non-modified peptide.
ImmunogenSynthetic peptide corresponding to aa30-38, CPATGGV(dimethyl-K)KP, of Histone H3 with a N-terminal Cysteine added for conjugation (KLH). The immunizing sequence is conserved from Tetrahymena to human.
SpecificityRecognizes human methylated Histone H3 (Lys36), Mr 17kD. Does not crossreact with peptides containing dimethyl histone H4 Lys20, nor Histone H3 Lys4, Lys9, or Lys27.
Species CrossreactivityMouse, chicken, Drosophilia and yeast.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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