| Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). |
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| Catalog # | H5110-17D |
| Applications | Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilutions | Western Blot: 0.01-0.1ug/ml detects methyl-histone H4 in HeLa acid extracts. Minimal crossreactivity with unmethylated recombinant Histone H4 is observed if used at these concentrations. |
| Immunohistochemistry: Stains Drosophila polytene chromosomes as reported by an independent laboratory. |
| Immunoprecipitation: Immunoprecipitates methly-Histone H4 from HeLa cell lysates as reported by an independent laboratory. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo |
| I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3J7 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa17-28 (RHR[di-meK]VLRDNIQGC) of human histone H4, conjugated to KLH. |
| Specificity | Recognizes human methyl-Histone H4, Mr 10kD. Species Crossreactivity: mouse, rat, and bovine. Broad species crossreactivity is expected based on sequence homology. Does not crossreact with yeast or Tetrahymena. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
