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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IH IC

Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Various post-translational modifications, such as acetylation and methylation, of core histones serve as regulators of gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Methylation also serves as a predominant form of post-translational modification of Histone H3, and has been linked to transcriptional regulation and epigenetic silencing via heterochromatin assembly (5). Lysines 4, 9 and 27 in the N-terminal tail of Histone H3 are the preferred sites of methylation.
Catalog #	H5110-23A
Applications	Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:20,000
	Immunohistochemistry: 1:100-1:250
	Immunocytochemistry: 1:250
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	6k210
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid.
Purity	Supernatant
Immunogen	A synthetic (Dimethyl-K)-peptide corresponding to residues surrounding Lys4 of Histone H3
Specificity	Recognizes human Histone H3 dimethylated on Lysine 4. Species Crossreactivity: rat. Predicted to crossreact with most species, based on sequence homology.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.