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You are here:Home » Antibodies » Abs to Histones » Anti -Histone H3, dimethylated, Lys4

Anti -Histone H3, dimethylated, Lys4

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IH IC
Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Various post-translational modifications, such as acetylation and methylation, of core histones serve as regulators of gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Methylation also serves as a predominant form of post-translational modification of Histone H3, and has been linked to transcriptional regulation and epigenetic silencing via heterochromatin assembly (5). Lysines 4, 9 and 27 in the N-terminal tail of Histone H3 are the preferred sites of methylation.
Catalog #H5110-23A
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:20,000
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:250
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k210
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid.
PuritySupernatant
ImmunogenA synthetic (Dimethyl-K)-peptide corresponding to residues surrounding Lys4 of Histone H3
SpecificityRecognizes human Histone H3 dimethylated on Lysine 4. Species Crossreactivity: rat. Predicted to crossreact with most species, based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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