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You are here:Home » Antibodies » Abs to Enzymes, Methyltransferase » Anti -Histone Methyltransferase SUV39H1

Anti -Histone Methyltransferase SUV39H1

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #H5205-05A
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-1ug/m detected SUV39H1 in U20S cells lysed in RIPA with 0.1% SDS and sonicated. U20S cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SUV39H1 (1ug/ml). Proteins were visualized using a goat antirabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide
(CQVDPVDMESTRMDSNFGLAGLPGSP) corresponding to amino acids 368-392 of mouse suppressor of variegation 3-9 homolog 1 (SUV39H1) with an N-terminal cysteine added for conjugation
purposes.
SpecificityRecognizes SUV39H1 Mr ~48kD. Species Crossreactivity: Human. Predicted to crossreact with rat and mouse based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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